epo  (R&D Systems)

Journal: Journal of Medical Biochemistry

Article Title: Randomized controlled trial of intraosseous access vs. intravenous access in traumatic hemorrhagic shock: Effects on inflammation, hematopoiesis, and coagulation

doi: 10.5937/jomb0-58956

Figure Lengend Snippet: Impairment of hematopoietic function. a) Comparison of changes in CD34 + , b) Comparison of changes in CFU-GM, c) Comparison of changes in BFU-E, d) Comparison of changes in CXCL12, e) Comparison of changes in EPO, f) Comparison of changes in TPO. Note: 'a' indicates P < 0.05 compared with T0, 'b' indicates comparison with T1, and 'c' indicates P < 0.05 compared with the IV group at the same time.

Article Snippet: The following indicators were analyzed: IL-1β (DLB50), IL-6 (D6050B), IL-10 (D1000B), HMGB1 (IC1690G), CXCL12 (MAB310), EPO (DEPRU0), and TPO (BAF288): These markers were quantified using enzyme-linked immunosorbent assay (ELISA) with commercially available kits (R&D Systems).

Techniques: Comparison

Journal: Frontiers in immunology

Article Title: Discovery and development of a safe and efficient COVID-19 mRNA vaccine, STP2104, using a novel capping library screening method.

doi: 10.3389/fimmu.2025.1571713

Figure Lengend Snippet: FIGURE 1 Evaluation of mRNA yield, capping efficiency, and potency of reporter genes using capping library screening. (a) SmartCap® (SC) library composition with ribose and base modification. In synthesized eGFP mRNA, (b) IVT products on 1% agarose gel, (c) capping efficiency of several 5′-cap analogues, (d) naked eGFP mRNA transfection-mediated fluorescence in HEK293T and Huh7 were evaluated. (e) In hEPO mRNA synthesis, mRNA yield, and potency were determined via hEPO ELISA assay in HEK293T and RD cell culture media. (f) In fLUC mRNA synthesis, yield, and potency were determined via luciferase assay in HEK293T and C2C12 cell lysate. (h) Female BALB/c mice were intramuscularly injected with PBS or a 5 mg dose of fLUC (Capping with SmartCap® library) mRNA LNPs. Luminescence was determined via IVIS (IVIS spectrum, PerkinElmer) up to 6 days p.i. Luminescence image from the whole body was measured over time for up to 6 days p.i. (h) Total flux of the whole body was plotted over time. Statistical analyses were performed using one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, FF ns: no significant).

Article Snippet: For mRNA transfection, HEK293T cells (1 × 106 cells/well) were plated in 6-well plates and cultured in GibcoTM Opti-MEMTM Reduced Serum Medium (Thermo Fisher Scientific) for 24 h. After 24 h of transfection, the fluorescence expressed by eGFP and hEPO in the cell cultures was analyzed by using an EVOS M5000 Microscope (Thermo Fisher Scientific) with a 10X scope and a Human Erythropoietin/EPO ELISA Kit (BioTechne® R&D System, Quantikine DEPRU0, Minneapolis, MN, USA), respectively.

Techniques: Library Screening, Synthesized, Agarose Gel Electrophoresis, Analogues, Transfection, Enzyme-linked Immunosorbent Assay, Cell Culture, Luciferase, Injection

Journal: International journal of molecular sciences

Article Title: Effects of Darbepoetin Alfa and Ferric Derisomaltose Plus Darbepoetin Alfa in Functional Iron-Deficiency Anemia.

doi: 10.3390/ijms26052203

Figure Lengend Snippet: Figure 7. Effects of ferric derisomaltose, darbepoetin alfa, and ferric derisomaltose plus darbepoetin alfa on EPO production and gene expression in F36E cells. F36E cells were treated with 10 ng/mL darbepoetin alfa, 100 ng/mL ferric derisomaltose, or a combination of 10 ng/mL darbepoetin plus 100 ng/mL ferric derisomaltose for 48 h. (A) EPO protein was analyzed in cell culture medium by ELISA. F36E erythroid cells were treated with 10 ng/mL darbepoetin alfa vs. 100 ng/mL ferric derisomaltose plus 10 ng/mL darbepoetin alfa for 48 h, and genes that were (B) upregulated or (C) downregulated were examined by KEGG pathway and gene ontology (GO) analyses.

Article Snippet: Concentrations of EPO in cell culture medium and human serum were determined using Human EPO DuosetTM ELISA and Human EPO Quantikine® ELISA kits (R&D Systems, Minneapolis, MN, USA).

Techniques: Gene Expression, Cell Culture, Enzyme-linked Immunosorbent Assay